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Multi-antigen vesicles encoding tolerogenic cytokines have reversed hyperglycemia and expanded regulatory T cells in non-obese diabetic mice (NOD mice), offering a targeted approach to autoimmune intervention that surpasses the scope of GLP-1RAs alone [432, 433]

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Because AF3 accurately placed the substrate in the correct register for the highly active SNAP25-cleaving proteases WT BoNT/E and D3, but not for the proteases evolved not to cleave SNAP25, evolved proteasesubstrate complexes were instead prepared by mutating the parent WT or D3 complex prediction to the evolved sequence