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Histological evaluation followed established methodologies, including the NAFLD Activity Score (NAS) for grading steatosis, inflammation, and hepatocellular ballooning, and the NASH Clinical Research Network (CRN) fibrosis staging system for quantifying fibrosis severity

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it is also implicated in the extracellular matrix formation, microtubule dynamics, and primary cilium maintenance, which are essential for cellular polarity and signaling

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