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It also plays a role in growth hormone replacement therapy, aiming to restore GH release to optimal levels
For Plp-staining cells were washed and stained with anti-Plp 1:500 (NB100-1608) (Novous Biologicals, Littleton, CO) overnight followed by anti-chicken-Cy3 (Thermo Fisher Scientific, Waltham, MA) and co-stained with CellMask and Hoechst (Thermo Fisher Scientific, Waltham, MA) for 5 min at +4 C
When administered together, the GH response is synergistic typically 2-5 times greater than either peptide alone because GHRH primes the somatotroph's secretory capacity while GHRP triggers the actual GH release burst
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