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NADPH regenerating system was prepared as follows: 1.7 mg/mL NADP, 7.8 mg/n L gIucose-6-phosphate and 6 units/mL glucoses- phosphate dehydrogenase were added to 10 mL 2% sodium bicarbonate and used HGF Binding The binding of 6-AH analogs to HGF was assessed by competition using a soluble binding assay

Fluorescent images were acquired using an inverted fluorescence microscope (Olympus Corp., Japan)

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Passing out from drinking too much means the drinker is unconscious and has consumed too much alcohol