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A major role of GSH in the cellular antioxidant defense network is to neutralize ROS produced during glycolysis

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note that the end of the Nco I site (CCATGG) overlaps by one base (G) with one of the priming sites

Preclinical models demonstrate that these compounds reduce reactive oxygen species, stabilize mitochondrial membrane potential, enhance mitophagy, and activate NRF2 dependent transcription, which translates into attenuation of ASD like behaviors in VPA, propionic acid, maternal immune activation, and BTBR paradigms (Yang et al., 2020