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The slides were incubated with primary antibodies in a Tris-buffered saline, 0.1% Tween 20 Detergent solution (TBST) overnight in a humidified chamber at 4C

Development 124 , 925936 (1997)

Whereas phosphodiesterase-5 (PDE-5) inhibitors like sildenafil and tadalafil operate peripherally increasing blood flow to genital tissue PT-141 bypasses vascular physiology entirely and acts directly on the brain