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After stimulation of indicated solution and inhibitors, the supernatant was removed and then rinsed thrice in phosphate-buffered saline (PBS, HyClone, #SH30256.01B)

Energy levels typically improve between weeks six and twelve

Astrocytes generate an inductive tightening influence on the endothelium through the production of sonic hedgehog (Shh) (Alvarez et al., 2011) and basic fibroblast growth factor (bFGF) (Sobue et al., 1999) that assist in forming a restrictive BBB by promoting endothelial expression of tight junction (TJ) proteins, occludin, claudins, and zonula occludens (ZO)

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