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Supercooling prevents the accumulation of free fatty acids and consumption of acyl-carnitines Storage-induced and oxidant stress-dependent activation of phospholipase A2 (PLA2) or PLA2-like enzymes such as peroxiredoxin 6 50,51 has been observed during RBC storage and represents a hallmark of storage-induced lipid peroxidation (Fig
Third, applying the method in the microplate reader is challenging due to the requirement for protein precipitation
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