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Conversely, indirect measurements investigate biomarkers that are present in collected samples, most commonly blood, that may be utilised for assessment of intestinal permeability

It is also used in PMLE, acne vulgaris, vitiligo, psoriasis, anti-aging etc

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The callose content was quantified using aniline blue staining, followed by fluorometric measurement with an F-7000 fluorescence spectrophotometer (Hitachi High-Tech Corporation, Tokyo, Japan)