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These findings emphasize that standardization of the extraction process is crucial to minimize matrix interference, thereby ensuring reliable assay performance

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TNF- stimulation-induced oxidative stress and downregulated claudin-5 and occludin expression in HBMEC co-cultured with astrocytes under ischemic conditions (Abdullah et al., 2+ /NF pathway in hCMEC/D3 cells inducing synthesis of the metalloproteinase MMP9, correlated with BBB degradation in several pathologies (Ding et al., via Ca 2+ -dependent signals, to decreased expression of TJs proteins and increased permeability (Propson et al., 2021)

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