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If you have a protocol that requires very frequent draws, it might be more prudent to reconstitute smaller amounts in separate vials

In many cases, proteins with relatively low sequence identity may still share highly conserved three-dimensional structural features and functional sites, allowing them to perform similar biological functions

Proline biosynthesis is a vent for TGFinduced mitochondrial redox stress

Subsequently, samples were concentrated to dryness in vacuum before dissolving in 150 L of 80% methanol (precooled at -20C), then centrifuged again at 13 500 g and 4C for 10 min to obtain the supernatant for high-performance liquid chromatography Ultimate 3000 (Thermo Fisher Scientific, USA)-tandem mass spectrometry Q Exactive Focus (Thermo Fisher Scientific, USA) (LC-MS/MS) analysis ( 2.3.2 Extraction of Total Metabolites All samples were thawed at 4C, and 100 L of each sample were pipetted to new 2 mL tubes