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[6] Crucially for the systemic story, they then moved into in vivo rodent models

7.3 Metabolic context in aging: gut microbiota, lipid mediators, and their interaction with exercise and amino acid supplementation Aging is accompanied by profound metabolic remodeling that influences musclevascular health and modulates the responsiveness to nutrients such as CIT and leucine, as well as to physical exercise

The vagal pathway of ghrelin action in the hypothalamus is integrated into the systemic (peripheral) pathway of ghrelin action

The samples were incubated with mouse primary monoclonal antibody, anti-Nestin 1:200 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), anti-GAP43 (1:500