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Proteomics: Analysis of the stability and metabolic degradation pathways of multi-agonist compounds

Strati F, Cavalieri D, Albanese D, De Felice C, Donati C, Hayek J, Jousson O, Leoncini S, Renzi D, Calabr A, De Filippo C

You will learn the exact temperatures required for short-term and long-term storage, why your container choice matters more than you think, how bacteriostatic water extends shelf life compared to sterile water, and which specific peptides demand special attention

Strains carrying an HA-tagged version of Adh2 were generated by similar approaches using pYM-N16 (EG103 pGPD-3HA-ADH2 and EG110 pGPD-3HA-ADH2 ) or pYM-N14 (EG103 ADH2-6HA and EG110 ADH2-6HA ) as templates and primers ADH2_S1 and ADH2_S4 or 5-GGCATACTTGATAATGAAAACTATAAATCGTAAAGACATAAGAGATCCGCTTAATCGATGAATTCGAGCTCG-3 (ADH2_S2) and 5-AGATGGAGAAGGGCCAAATTGCTGGTAGATACGTTGTTGACACTTCTAAACGTACGCTGCAGGTCGAC-3(ADH2_S3), respectively