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The Eppendorf tube was then centrifuged at 7,000 g for 5 min, and the pellet was dissolved in 0.6 l of preservation medium (0.5 mM EGTA, 3 mM MgCl 2 6H 2 O, 60 mM K-lactobionate, 20 mM Taurine, 10 mM KH 2 PO 4 , 20 mM HEPES, 110 mM sucrose, 1 gl 1 BSA, 20 mM histidine, 20 M vitamin E succinate, 3 mM glutathione, 1 M leupeptin, 2 mM glutamate, 2 mM malate, and 2 mM Mg-ATP

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