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The peptide mixture was loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 m 2 cm, nano Viper C18) connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 m inner diameter, 3 m resin) in buffer A (0.1%formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology

Sermorelin Peptide is a synthetic analog of Growth Hormone-Releasing Hormone (GHRH)

Scalp skin tolerates different levels than facial skin, and the target tissues (hair follicles) respond to different signaling intensities

Numbers link components of the pathway to temporal plots depicted in the lower part of the Figure