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Blood samples were centrifuged approximately 60 minutes after collection, and serum cortisol levels were analyzed in an accredited diagnostic laboratory on a Cobas e411 analyzer using a competitive electrochemiluminescence immunoassay (Roche Diagnostics, Rotkreuz, Switzerland) with a coefficient of variation of 3.6% at 793 nmol/L

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Sermorelin is a truncated analog of an endogenous releasing factor, while GHRP-6 is a synthetic hexapeptide examined for its interaction with receptor systems distinct from those targeted by releasing-factor fragments

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