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Finally, cells were observed using a Leica TCS SP8 confocal laser scanning microscope, acquiring images using an excitation wavelength of 488 nm with FITC emission wavelength between 500 and 580 and DAPI emission wavelength between 405 nm and 410490 nm

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High glucose drives renal cell damage (podocytes, TECs, etc.) (118), and ferroptosis (a non-apoptotic cell death modality) is a key mediator of DN progression