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Firstly, 3696 parallel linear models were constructed, re-setting the reference factor to compare all possible permutations of diagnostic class, with peptide quantification as outcome variables and categorical demographic information as explanatory variables: Secondly, 3696 parallel linear models were constructed with peptide quantification as outcome variables, and continuous demographic information as explanatory variables: This similar second model was constructed to allow consideration of nuanced interparticipant differences that may be lost by stratifying individuals into discrete classes

As both these approaches targeted the active site, we shifted to developing two high-throughput, robust, yeast-based assays that were active site independent

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