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Mass filtering was performed by the quadrupole with 0.7 m/z transmission window, followed by CID fragmentation in the linear ion trap with 35% normalized collision energy in rapid scan mode and a parallelizable time option was selected

TB-500 is a synthetic 17-amino-acid fragment (Ac-SDKPDMAEIEKFDKSKLKKTET-NH2 is the full TB-4 sequence

Hence, the results of the meta-analysis may indicate reduced vagal modulation in ME/CFS, along with increased sympathetic modulation of heart rate (329)

The success of this approach in modulating both energy metabolism and epigenetic regulation suggests that other metabolic nodes may offer similar opportunities for dual-pathway intervention