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Step 5: Monitor How Your Body Responds Watch for your hunger returning, any changes in blood sugar if you have diabetes, and how your energy and mood shift in the first few weeks

Tissue sections were incubated with primary mouse antibodies FITC anti-CD192 (1:100 dilution) (CCR2) (BioLegend, 150607), PE anti-CD11b (1:200 dilution) (BioLegend, 101208), anti-CD68 (1:200 dilution) (BioLegend, 137001), anti-F4/80 (1:200 dilution) (BioLegend, 123101), and anti-IL-6 (D5W4V) (1:200 dilution) (Cell Signaling 12912S) overnight, stained with secondary antibody (for CD68, F4/80, IL-6) (Invitrogen SA5-10018, A21434 and A11036)

Possible disadvantages may include gastrointestinal side effects such as nausea, bloating, constipation, or diarrhea, particularly during the early stages of treatment