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Key Takeaway: Skin brightening occurs when glutathione shifts the body's pigment production from dark melanin (eumelanin) to lighter melanin (pheomelanin) by regulating the tyrosinase enzyme

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Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 340 nm at 25C Precision pipettes to deliver 2 L to 1 mL volumes Multi-channel pipettes to deliver 20 L to 200 L volumes Tubes to prepare sample dilutions 15 mL conical tubes Incubator at 25C Deionized water Assay Procedure Summary Prepare Working Solution