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Twenty thousand cells per well were processed in a BD Accuri C6 flow cytometer (BD Biosciences, San Jose, CA, United States) and analyzed by FlowJo v

Because of the simplicity of traditional cell culture plates or transwell filters, they are useful for screening multiple compounds and looking at many mechanistic outputs, while the other models are often used for more specialized readouts

doi: 10.3390/foods12091750 51 MaNBTonNMNLeNL

This allowed them to model reward-prediction error related activity and expected value assigned to conditioned stimuli, instead of relating ghrelin to reward responses per se