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These cells were cultured in Dulbeccos modified Eagles medium supplemented with 10% fetal bovine serum (Gibco BRL, USA) and were maintained in a humidified incubator containing an atmosphere of 5% CO 2 at 37 C

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Previously, our lab has constructed transposon mutant libraries in K56-2, a multidrug-resistant ET12 epidemic lineage clinical isolate 26 , to identify the essential genome 27 and characterise targets and mechanisms of action for antimicrobials 28,29,30

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