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This dual-enzyme cascade is widely present in the cytoplasm and is highly sensitive to oxidative stress

The phenylacetyl group acts as a protective shield, preventing premature breakdown in the digestive system and allowing significantly higher cellular uptake compared to standard reduced glutathione

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However, it is important to note that curcumin has also been identified as a PAINS, exhibiting behaviors such as redox cycling, metal chelation, aggregation, and fluorescence interference that can artificially inflate its apparent bioactivity in vitro (Nelson et al., 2017)