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The vehicle and treated fractions tubes were resuspended in the vehicle and treated solution each and incubated at 37C for 20 min

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Its a small detail, but it makes a big difference in treatment effectiveness over time

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Human data followed: a prospective, double-blind RCT in Diabetes Care found that daily administration of Lactobacillus reuteri SD5865 increased glucose-stimulated GLP-1 release by 76% compared to placebo, with corresponding increases in insulin secretion

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