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After one month of their habitual diet, which was designed to reflect serum L-Cn fluctuation, the volunteers were shifted to a weeklong beef-rich diet (around 220 g cooked beef during each lunch and dinner, equivalent to an estimated 330 mg L-Cn per day)

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Add the elution buffer to the column, cap both ends, and incubate for a few minutes before uncapping and dripping your elution out of the bottom (Figure 4)

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