Anti-FAR1 (Novus Biologicals, LLC, NBP189847, 1:1000 dilution), anti-AGPAT3 (abcam, ab211435, 1:500), monoclonal Anti--Actin antibody (Sigma, A5441, 1:5000), anti-ACSL4 (Santa Cruz Biotechnology, sc-365230, 1:1000 dilution), anti-ferritin (abcam, ab75973, 1:2000), anti-ATXN7L3 (abcam, ab99947, 1:1000)
Gastroenterology 156, 14401454.e1442
The female mice (homozygous for Hba tm1(HBA)Tow and heterozygous for Hbb tm2(HBG1,HBB*)Tow and Hbb tm3(HBG1,HBB)Tow [h/h:: A / S ], sickle-cell trait mice) and male mice (homozygous for both Hba tm1(HBA)Tow and Hbb tm2(HBG1,HBB*)Tow [h/h:: S / S ], sickle SCD mice) were bred to generate SCD mice
Subcutaneous injection into abdominal fat or near the site of injury (as commonly practiced by self-experimenters) is the most reported approach, but this is not a clinical protocol