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Then, 50 l of diluted Microparticle Cocktail was added to each well, the plate was covered and incubated for 2 h at room temperature on a shaker at 300 r.p.m

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e , Left, volcano plot showing relative fold change (log 2 ) in whole cell metabolite abundance versus log 10 (P value) from HEK293T cells expressing a sgRNA targeting intergenic region or sgRNA targeting SLC33A1

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