Culture medium consisted of Dulbeccos Modified Eagle Medium (DMEM): Hams F-12 Nutrient Mixture (1:1) supplemented with 100 U/mL Penicillin and 100 g/mL Streptomycin, 2 mM Glutamine, 0.5x N2 supplement, 2.6 ng/mL insulin, 2 nM dexamethasone, 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF)
Ingredients: Non-Dairy Creamer, Reduced L-Glutathione, N-Acetyl Cysteine, Tranexamic Acid, Alpha Lipoic Acid, Ashwagandha Extract, Hibiscus Extract, Licorice Root Extract, Vitamin C, Evening Primrose, Stevia
This 1:1 ratio is not arbitraryit balances solution viscosity (too concentrated and the peptide may precipitate
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