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As outlined above, studies on non-glutathione disulfide substrates unfortunately regularly lack more detailed kinetic analyses, first, to confirm the efficient turnover of the substrate candidates and, second, to validate the dithiol mechanism

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In the model proposed by Lee, homocysteine is shunted away from the methionine synthesis pathway and toward the GSH synthesis pathway, creating a relative deficiency of methyl donors in order to keep up with increased demand for the glutathione needed for POP conjugation ( Fig 3 )