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Tissues were lysed by transferring them to pre-filled bead mill tubes (Fisher Scientific, 15-340-154) containing lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail and homogenized using a bead mill (VWR) for 10 s

Kennedy and Trump are also cutting funding for basic medical research, slashing the budget of the National Institutes of Health, which is responsible for many of our medical breakthroughs and drug development, including in psychiatry