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However, widespread urticaria, mucosal involvement, or systemic symptoms such as dyspnea warrant immediate evaluation
Kadouh H, Chedid V, Halawi H, et al
The concentrations of protein extracts were determined by using the BCA Protein Assay Kit (Thermo Fisher Cat #23225), then protein samples were mixed with 3 loading buffer and loaded in equal amounts into the wells of 412% Bis-Tris protein gels (Bio-Rad) to transfer the proteins to PVDF membranes (Millipore)
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