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Specific research questions (angiogenesis-dominant or cell-migration-dominant models) might call for different proportions, in which case separate vials let you set the ratio yourself

10.1038/s41591-022-02026-4 [DOI] [PMC free article] [PubMed] [Google Scholar] 72

The study of spontaneous metastasis is hindered since the required interval for spontaneous metastasis to occur in vivo takes much longer than the commonly used intravenous inoculation of tumor cells, in addition animal wellness rules in almost all institutions, frequently discourages the practice to keeping tumor-bearing mice for a long period of time