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After 10 days, cells were fixed and stained with 70% ethanol, 0.4% (w/v) methylene blue and detained with distilled water

Prolotherapy is particularly effective for: Ligament laxity and instability (knees, ankles, shoulders) Tendinopathies and tendon tears Chronic low back pain from ligament weakness Joint instability Sacroiliac joint dysfunction Conditions that have failed to improve with other conservative treatments PRP (Platelet-Rich Plasma) Therapy PRP therapy uses a concentrated preparation of the patients own blood platelets drawn, processed, and re-injected into the damaged area

Alcohol and heat: Avoid for at least 48 hours

This is known as anemia