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To confirm GSTP1 knockdown in A2780DPP cells, we isolated individual puromycin-resistant colonies 24 h after colony formation using cloning cylinders, and extracted sufficient RNA to confirm GSTP1 knockdown by qRTPCR analysis (Figure 3B)

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Linaclotide for irritable bowel syndrome with constipation: a 26-week, randomized, double-blind, placebo-controlled trial to evaluate efficacy and safety