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For oxygen consumption measurement, the mitochondria were resuspended in MiR05 medium (0.5 mM EGTA, 3 mM MgCl 2 , 60 mM lactobionic acid, 20 mM taurine, 10 mM KH 2 PO 4 , 20 mM HEPES, 110 mM Sucrose, 0.1% BSA, adjusted to pH 7.1 with KOH)

Sermorelin is a 29-amino-acid fragment of native GHRH that was FDA-approved under the brand name Geref for pediatric growth-hormone deficiency before being voluntarily withdrawn from the U.S