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Activity was monitored by the formation of a fluorescent product (coumarin) generated by a two-step coupled reaction that involved deacetylation of substrate by SIRT-1 followed by secondary release of the fluorophore
In rodent models, reported effects in tendon, ligament, muscle and gastrointestinal tissue have been attributed to modulation of the VEGFR2 / nitric oxide pathway, upregulation of growth-hormone receptor expression in fibroblasts, and effects on FAKpaxillin signalling that influence cell migration
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