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Materials and Methods As of March 27, 2025, there is one experimental structure determined by X-ray diffraction of the semaglutide backbone in complex with GLP-1R ECD (PDB ID: 4ZGM [24]), which is used here as the template for high-throughput [28] structural modeling by Modeller [29] and ligand-receptor binding affinity (K d ) calculations by Prodigy [30, 31]