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The YAK1 -KD (kinase dead, p.K398R) and MCK1 -KD (p.K68R) mutants were created by site-directed mutagenesis (QuickChange Lightning, Agilent technologies, Santa Clara, CA, USA) according to the manufacturer instructions using primers listed in Supplementary Table 2
no formal PK studies published) ~2 hours (1.3h in SBS patients
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