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Patients under the age of 18 will require a GP appointment before being able to proceed with an injection

Nakayama, 2002) leading to stimulation of all 5-HT receptors present in these areas

Reverse Transcription-Quantitative Polymerase Chain Reaction The total RNA content was isolated from cells or the tumor using TRIzol reagent (Invitrogen), treated with DNase I (Thermo Fisher Scientific, Cat# EN0521), and then reverse transcribed into cDNA using a Maximal First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Cat# K1622)