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These inhibitors can be broadly categorized based on their mechanisms of action, including iron chelators that limit the availability of redox-active iron, lipid peroxidation inhibitors that suppress oxidative stress, and activators of endogenous antioxidant pathways such as nuclear factor erythroid 2related factor 2 (NRF2) and GPX4 stabilization [10]

The Acsl4 conditional knockout (cKO) model ( Acsl4 fl/fl Ksp Cre ) was engineered through crossbreeding of Acsl4 fl/fl mice with Ksp Cre transgenic strains, while the Fsp1 fl/fl Ksp Cre cKO mice were generated using analogous breeding strategies

This dual role mechanism enhances cellular antioxidant capacity, positioning NAC as a promising therapeutic candidate for managing Hg-induced lung toxicity