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In vitro treatment of T cells To establish the Tsen-cell-induction model, 1 10 6 /ml splenic CD8 + T cells of tumor-bearing mice (tumor-bearing for 2 w) were seeded in 12-well plate (6 10 6 /well) and cocultured with ID8 cells (at a ratio of 8:1) and MATES at different concentrations for 48 h