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An Ultimate 3000 UHPLC (Dionex) was coupled to the Q Exactive-Mass plus spectrometer (QE-MS, Thermo Scientific) for metabolite separation and detection

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Besides being a potential source of more reactive free radicals via Fenton reaction, physiological generation of H 2 O 2 fulfills a second messenger role and can be transported across membranes by aquaporins, a family of proteins that act as peroxiporins (Sies, 2014)

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