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After neutralizing the mixture with NaOH it was subjected to ultracentrifugation through a membrane with a molecular weight cut off of 50,000 Da

10.1111/j.1369-1600.2006.00012.x 53 SayehmiriF.SamadianM.MohamadkhaniA.TafakhoriA.HaghighatS.RahmatianA.et al (2022)

( b ) MAD participant flow chart (CONSORT diagram)

Subsequent Gene Ontology (GO) enrichment analysis delineated marked perturbations in key biological processes, including cellular processes (e.g., membrane integrity maintenance), metabolic reprogramming (particularly carbohydrate and lipid metabolism), cellular anatomical entity organization (notably gut epithelial junction complexes), catalytic activity modulation (encompassing oxidoreductases and hydrolases), and molecular binding dynamics (e.g., receptor-ligand interactions) (Figure 2G)