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The data from only the mice completing the protocol were analyzed since the most of the Pdx1-Cre;Trp53 R172H ;Rb f/f mice developing insulinoma die within 2 weeks after presenting with hypoglycemia as previously reported 22

After first reconstitution (puncturing the rubber stopper with a sterile needle to introduce bacteriostatic water), the vial's internal environment is exposed, though the rubber stopper reseals when the needle is removed

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