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Tirzepatide dosing protocol Tirzepatide uses a longer, more gradual titration over 20 weeks to reach the maximum dose: Weeks 1-4: 2.5mg once weekly Weeks 5-8: 5mg once weekly Weeks 9-12: 7.5mg once weekly Weeks 13-16: 10mg once weekly Weeks 17-20: 12.5mg once weekly Week 21 onward: 15mg once weekly (maximum maintenance) Not everyone needs to reach 15mg

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To measure lysosomal dynamics, cells were incubated with 50nM LysoTrackerTM Deep Red (ThermoFisherScientific), and Hoechst 33342 in HBSS for 40 minutes and then imaged using Zeiss LSM 880 confocal microscope where the 405 nm, and 647 nm laser line were used to excite Hoechst 33342 and LysoTrackerTM Deep Red, respectively

TakeshimaMMiyazakiIMurakamiSKitaTAsanumaM.L-theanine protects against excess dopamine-induced neurotoxicity in the presence of astrocytes