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Glutathione (reduced and oxidised) concentrations were assayed by HPLC with fluorimetric detection of oxidised and reduced glutathione, according to the method proposed by Martin & White (Reference Martin and White1991)

Before embryo injection, chemically modified single guide RNAs (sgRNAs) were tested for editing efficiency in mouse Neuro-2A cells [American Type Culture Collection (ATCC)] stably expressing Streptococcus pyogenes ( Sp Cas9) and assayed by targeted next-generation sequencing as previously described ( 90 , 91 )