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Chromatographic separation was carried out using an UltiMate 3000 HPLC system (Thermo Fisher Scientific, Rockford, IL, USA) fitted with a YMC-Triart PFP column (3 mm particle size, 12 nm pore size, 150 2.1 mm

In vitro studies using primary rat striatal neurons indicate that micromolar concentrations of 3-HK induce toxicity primarily via hydrogen peroxide formation [155]

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