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To explore the functional role of FOXO4 in senescence, its expression was silenced using siRNA in TM3 Leydig cells prior to H2O2-induced senescence
Interestingly, when we optimized the cell sorting procedure by using free settling to replace magnetic separation, the EGFP positive cell percentage could be elevated from 20% to over 95% in K-562 cells for both GST-EGFP-GPI DAF and GST-EGFP-GPI BY55 (Figure 3C)
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