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MITOCHONDRIAL ROS GENERATION AND DEFENSE Reactive oxygen species can be generated in several intracellular sites, including cytosol, peroxisomes, plasma membrane, and ER

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Lipid peroxidation during ferroptosis proceeds as follows: (1) the initial reaction occurs between ROS (e.g., hydroxyl radicals), that seem to be derived from NADPH oxidase rather than mitochondrial ETC, at least under some circumstances (Dixon et al., 2012), and hydrogen atoms in unsaturated lipids, typically polyunsaturated fatty acids (PUFAs)